Downregulation of chalcone isomerase in Petunia hybrida: profound effects on floral morphology and transcriptional remodeling of the flavonoid biosynthetic pathway.

ABSTRACT:

Background

Development ornamental varieties with enhanced floral traits and distinctive characteristics is a central goal in floriculture. Anthocyanins, the principal flavonoid pigments in floral tissues, are synthesized via the well-characterized flavonoid biosynthesis pathway. Chalcone isomerase (CHI) catalyzes the first committed reaction in the flavonoid biosynthetic pathway.

Method

In this study, we investigated the effects of CHI suppression via RNA interference (RNAi) in three Petunia hybrida cultivars exhibiting distinct petal colors. Leaf discs from three Petunia hybrida lines exhibiting distinct petal colors (blue, pink, and purple) were inoculated with Agrobacterium tumefaciens harboring the recombinant plasmid pBI121 containing the RNAi construct targeting Phchi.

SUBMITTER: Keykha Akhar F 

PROVIDER: S-EPMC12573930 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature