The Chinese herb component salvianolic acid B induces copper-mediated reactive oxygen species generation and oxidative DNA damage.

ABSTRACT:

Background

Salvianolic acid B (Sal B), a natural polyphenol with potential therapeutic applications, has been reported to induce reactive oxygen species (ROS) generation. However, its underlying mechanism has not yet been fully elucidated. In this study, we investigated copper-mediated oxidative DNA damage induced by Sal B.

Results

Sal B significantly increased the level of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in HL-60 cells, but not in H₂O₂-resistant HP100 cells. The formation of 8-oxodG was inhibited by a Cu(I)-specific chelator. These results suggested that Cu(I) and H₂O₂ play critical roles in this process. In calf thymus DNA, Sal B induced 8-oxodG formation in the presence of Cu(II), which was markedly enhanced in the

SUBMITTER: Kobayashi H 

PROVIDER: S-EPMC12574035 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature