Soil-based environmental DNA enables detection of Oncomelania hupensis quadrasi and Schistosoma japonicum microhabitats for schistosomiasis japonica surveillance and control in the Philippines.

ABSTRACT:

Background

Schistosomiasis japonica, caused by Schistosoma japonicum, remains a significant public health concern in the Philippines, where 12.4 million people are at risk due to persistent transmission in endemic regions. The distribution of schistosomiasis is closely linked to the distribution of its snail intermediate host. This study aims to assess the potential of using soil samples to detect the environmental presence of Oncomelania hupensis quadrasi and Schistosoma japonicum.

Methods

This cross-sectional observational study utilized a soil-based environmental DNA (eDNA) detection system for simultaneous detection of O. h. quadrasi, and S. japonicum mitochondrially encoded cytochrome c oxidase subunit 1 gene in multiplex quantitative real-time PCR and digital PCR platf

SUBMITTER: Valencia JE 

PROVIDER: S-EPMC12574143 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature