The microRNAs landscape of luminal B breast cancer cells in a three-dimensional microenvironment.

ABSTRACT:

Background

Three-dimensional (3D) culture captures key features of tumor architecture and microenvironmental signaling. Because microRNAs (miRNAs) are central post-transcriptional regulators, we asked whether 3D growth would reveal disease-relevant lncRNA-miRNA-mRNA programs in BT-474 luminal breast cancer cells.

Methods

BT-474 cells were profiled with GeneChip miRNA 4.0 microarrays to compare 3D versus 2D conditions. We then performed an integrative analysis consisting of: (i) extracting miRNA-mRNA interactions from miRNet and ENCORI (predicted and curated) for the differentially expressed miRNAs; (ii) identifying inverse miRNA-mRNA pairs (miRNA downregulated with target mRNA upregulated, or the reverse) using public mRNA data from BT-474 3D versus 2D cultures so that pairs

SUBMITTER: Nunez-Olvera SI 

PROVIDER: S-EPMC12574175 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature