Nanoscale Precise Stamping of Biomolecule Patterns Using DNA Origami.

ABSTRACT: Understanding the importance of ligand patterning in biological processes requires precise control over molecular positioning and spacing. While DNA origami structures offer nanoscale precision in biomolecule arrangement, their biological applications are limited by challenges related to their structural stability, scalability, and surface area. Here, we present a straightforward and rapid DNA origami stamping technique for transferring nanoscale oligonucleotide patterns onto surfaces, visualized using DNA-PAINT super-resolution microscopy to quantitatively assess the stamping efficiency and precision across different stamp types. Unlike traditional top-down methods that require specialized equipment, our technique provides an accessible, self-assembled platform for surface patterning, wit

SUBMITTER: Teodori L 

PROVIDER: S-EPMC12574213 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature