Ex vivo lung-organoid model for aberrant basaloid cell induction and activation.

ABSTRACT:

Background

Pulmonary fibrosis (PF) is a severe lung disease characterized by the destruction of lung architecture resulting from chronic epithelial injury. The PF microenvironment induces PF-specific epithelial cells, such as aberrant basaloid cells (ABCs). However, limited experimental models capable of inducing and activating PF-specific epithelial cells hinder the understanding of their roles.

Methods

To address the lack of experimental models, in this study, we developed an ex vivo murine lung-organoid model designed to induce and activate ABCs. The organoids were subjected to bleomycin (BLM) stimulation. Dose-dependent reductions in number and size, structural disorganization, and transcriptomic changes were assessed following stimulation. Single-cell RNA-sequencing (sc

SUBMITTER: Wu B 

PROVIDER: S-EPMC12574287 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature