Single-Cell Splicing Isoform Atlas of the Adult Human Heart and Heart Failure.

ABSTRACT:

Background

Alternative splicing plays crucial roles in normal heart development and cardiac disease by influencing protein-coding sequences, functional domains, and molecular networks. However, a detailed characterization of the human heart isoform landscape remains incomplete.

Methods

Leveraging long-read single-nucleus RNA sequencing and computational analysis, we dissected full-length isoform heterogeneities, expression patterns, and usage shifts across cell types, cell states, and cardiac conditions of the adult left ventricle. We applied in silico approaches to assess the functional relevance of identified isoforms; validated isoform compositions of representative cardiac genes using reverse transcription quantitative polymerase chain reaction and targeted amplicon sequ

SUBMITTER: Pan T 

PROVIDER: S-EPMC12574690 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature