CD40-CD154 Interactions Induced Progressive Neurodegeneration in Acute Ocular Hypertension Mice.

ABSTRACT:

Purpose

This study aimed to investigate the role and mechanism of CD40-CD154 interaction in acute ocular hypertension-induced progressive retinal inflammation and damage.

Methods

Transmission electron microscopy was used to observe the optic nerve of C57BL/6 mice at different time points of acute ocular hypertension and the sham group, and Western blotting was used to examine the relative expression of CD40 and CD154 at different time points. Knocking down CD40 and CD154 with CD40 and CD154 small interfering RNA, respectively, glaucomatous neural damage was assessed by using axon counts and observed by transmission electron microscopy imaging of the optic nerve. Immunofluorescence was used to determine the localization of CD154 and CD40 in the retinas of mice and flow cytome

SUBMITTER: Tang J 

PROVIDER: S-EPMC12574744 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature