SIRT1/PGC-1α/Mfn2 pathway regulates mitochondrial homeostasis in VSMC to attenuate aging-related vascular calcification.

ABSTRACT: Aging-related vascular calcification is closely associated with the development and progression of arterial diseases in elderly patients. Studies have shown that mitochondrial fusion/fission imbalance plays a crucial role in the formation of aging-related vascular calcification, and upregulation of silent information regulator 1 (SIRT1) blocks mitochondrial damage thereby protecting vascular smooth muscle cells (VSMCs). Our study aimed to explore whether SIRT1 can inhibit the vascular calcification and its potential molecular mechanism. VSMCs were used to establish the vascular aging model, and confirmed by β-galactosidase staining. The overexpressed SIRT1 and si-PGC-1α were transfected into VSMCs and verified by RT-qPCR or Western Blot. Proteins relevant to vascular calcification were det

SUBMITTER: Wu C 

PROVIDER: S-EPMC12575675 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature