Rutin ameliorates sevoflurane-induced neurotoxicity by inhibiting microglial synaptic phagocytosis through the complement pathway.

ABSTRACT: Repeated neonatal exposure to sevoflurane may impair synapses and lead to developmental neurobehavioral issues. In this study, we assessed the therapeutic effects of rutin on sevoflurane-induced neurotoxicity. The mice were exposed to 3% sevoflurane for 2 h daily on postnatal days (PNDs) 6, 8, and 10. The Morris water maze was used and an open field test was performed to assess the memory and anxiety-like behavior of the mice on PNDs 37-42. The effects of rutin on the complement cascade and microglial synaptic elimination in the hippocampus of mice were validated by Western blotting, real-time RT qPCR, morphological analysis, and immunohistochemistry examinations. The results showed that rutin effectively alleviated cognitive dysfunction and synaptic impairment in sevoflurane-treated mice.

SUBMITTER: Tian X 

PROVIDER: S-EPMC12575870 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature