Electron fourier ptychography for phase reconstruction.

ABSTRACT: Exit wavefunction reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive materials. We demonstrate that the phase of the exit wave can be reconstructed to high resolution using a modified iterative phase retrieval algorithm from data collected in an alternative optical geometry. This method achieves a spatial resolution of 0.63 nm at a fluence of 4.5 × 10² e^-/nm², as validated on Cry11Aa protein crystals under cryogenic conditions. Notably, this method requires no instrumental modifications, is straightforward to implement, and can be seamlessly integrated with existing data collection software

SUBMITTER: Zhao J 

PROVIDER: S-EPMC12575875 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature