M6A Methylation-Modified hsa_circ_0044226 Attenuates Pulmonary Fibrosis by Suppressing Ferroptosis.

ABSTRACT: Idiopathic Pulmonary fibrosis (IPF) is a debilitating lung condition marked by chronic progression and reduced lung function. This study aimed to explore the functional role and regulatory mechanism of circular RNA circ_0044226 in the pathogenesis of pulmonary fibrosis. The expression of circ_0044226 was analyzed in peripheral blood and TGF-β1-stimulated RLE-6TN cells using qRT-PCR. The cellular location of circ_0044226 was analyzed using FISH. The expression levels of circ_0044226 and METTL3 were evaluated in cells and clinical samples. Protein levels of GPX4 and FTH1 were examined using Western blot, while the levels of MDA, Fe²⁺ and ROS were determined using commercial kits. The association of circ_0044226 with m6A modifications was validated by Methylated-RNA immunoprecipita

SUBMITTER: Qi F 

PROVIDER: S-EPMC12576025 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature