Identification of novel protein biomarkers of macrophage polarization using comparative proteomic analyses of murine primary macrophages.

ABSTRACT: Macrophages comprise the first line of host responses against injury and pathogens and therefore are critically engaged in tissue repair, host defense, and homeostasis maintenance. Depending on the surrounding microenvironment, macrophages polarize into a wide spectrum of immunophenotypes with 2 extreme opposite ends-proinflammatory M1 and anti-inflammatory M2. Elucidating the biochemical bases of distinct macrophage immunophenotypes, as well as discriminating between these phenotypes, are paramount to understanding the contributions of macrophage subpopulations to health and diseases. In this study, murine bone marrow-derived macrophages were treated with LPS or IL-4 to induce the M1/M(LPS) or M2/M(IL-4) state, respectively. Comparative proteomic analyses demonstrate that M1 and M2 macrop

SUBMITTER: Liu B 

PROVIDER: S-EPMC12576133 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature