Advance in the assembly of the plant mitochondrial genomes using high-throughput DNA sequencing data of total cellular DNAs.

ABSTRACT: The assembly of plant mitochondrial genomes presents unique challenges due to difficulties in isolating mitochondrial DNA (mtDNA) and plant mitochondrial genome characteristics, such as low interspecific conservation; sequence sharing among mitochondrial, nuclear and plastid DNAs; and complex structural variations. Our laboratory has sequenced and assembled a dozen plant mitochondrial genomes, testing various strategies and identifying numerous assembly issues. This review compared the advanced methods and tools for plant mitochondrial genome assembly, categorizing assembly algorithms into three groups: (1) reference-based, (2) de novo and (3) iterative mapping and extension. The performance of 11 software tools used most frequently over the past 5 years (GetOrganelle, Velvet, NOVOPlasty,

SUBMITTER: Ni Y 

PROVIDER: S-EPMC12576441 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature