Toward Translation of Cowpea Mosaic Virus Intratumoral Immunotherapy With a Scalable Production Process.

ABSTRACT: The plant virus cowpea mosaic virus (CPMV) is being developed as a drug candidate for intratumoral immunotherapy for solid tumours. Data from preclinical studies in mice and rats as well as clinical studies in canine cancer patients demonstrate potent efficacy and an excellent safety profile. Toward Investigational New Drug (IND)-enabling studies, a scalable and Good Manufacturing Practice (GMP)-compatible manufacturing process must be developed. Therefore, here, we focused on the downstream process and developed a scalable purification process. We systematically screened the following processes: acidic extraction conditions making use of the virion pH stability, ultrafiltration making use of the nanoparticle character and the virion size, and finally ion exchange chromatography based on t

SUBMITTER: Opdensteinen P 

PROVIDER: S-EPMC12576442 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature