The 14-3-3 Protein SlTFT1 Accelerates Tomato Fruit Ripening by Binding and Stabilising YFT1 in the Ethylene Signalling Pathway.

ABSTRACT: Ethylene plays a pivotal role during the fruit ripening process in tomato (Solanum lycopersicum). Previously, we have reported that the tomato EIN2-like protein YELLOW-FRUITED TOMATO1 (YFT1), a core component in the ethylene signal transduction pathway, exerts a critical regulatory function in the tomato fruit ripening process. However, the molecular mechanism of YFT1-mediated ethylene signalling during tomato ripening still remains largely unknown. In this study, we performed yeast two-hybrid screens of a cDNA expression library from tomato fruits and obtained a new YFT1-interacting protein, SlTFT1 (TOMATO FOURTEEN-THREE PROTEIN 1), and confirmed their interaction in vitro and in vivo. Further bimolecular fluorescence complementation (BiFC) assays indicated that SlTFT1 can specifically bi

SUBMITTER: Wen T 

PROVIDER: S-EPMC12576456 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature