A GhBGH2-GhGLK1 Regulatory Module Mediates Salt Tolerance in Cotton.

ABSTRACT: Soil salinisation, exacerbated by climate change and human activities such as irrigation mismanagement, improper land use and excessive fertilisation, has become a major constraint on global crop production by disrupting fundamental metabolic processes like seed germination and photosynthesis. In our previous work, transcriptome sequencing of salt-tolerant and salt-sensitive cotton germplasms identified Gh_D04G136300, a negative regulatory gene downregulated in salt-tolerant and salt-sensitive materials. Phylogenetic analysis revealed its closest homologue to be AtBGH2, leading to its designation as GhBGH2. Virus-induced gene silencing (VIGS) demonstrated that GhBGH2 silencing enhanced salt tolerance. To further validate its function, we generated bgh2 knockout mutants via CRISPR/Cas9, whi

SUBMITTER: Wang P 

PROVIDER: S-EPMC12576457 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature