FKBP5 Regulates Osteogenesis of Human iPSC-Derived Mesenchymal Stem Cells via FKBP5-AKT-FOXO1 Pathway.

ABSTRACT: The induced pluripotent stem cells derived mesenchymal stem cells (iMSCs) have shown great promise for bone tissue regeneration in critical-sized calvarial defects. Still, the roles of FKBP5 in its osteogenesis are rarely known. It was observed that FKBP5 increased rapidly in iMSCs following osteogenic differentiation. To elucidate its role, FKBP5 was knocked down or overexpressed by lentivirus infection. Interestingly, the down-regulation of FKBP5 impaired the osteogenesis of iMSCs, whereas the up-regulation of FKBP5 promoted it. Proteomics analysis of iMSCs/oeFKBP5 and iMSCs/oeNC revealed that the protein variances are enriched in several signalling pathways associated with osteogenesis. Notably, the PI3K-AKT signalling pathway was enriched highly at both D4 and D14. Co-immunoprecipitati

SUBMITTER: Tian XY 

PROVIDER: S-EPMC12576591 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature