Birefringence microscopy enables rapid, label-free quantification of myelin debris following induced cortical injury.

ABSTRACT:

Significance

Myelin breakdown is prevalent in a range of neurodegenerative diseases, aging, and following various forms of trauma. Yet, current imaging techniques have limited capacity for large-scale study of myelin structural damage. A high-throughput, quantitative imaging method would greatly enhance our understanding of myelin degradation in different contexts.

Aim

We aim to establish birefringence microscopy (BRM) as a high-throughput, label-free imaging technique for large-scale, quantitative assessment of myelin pathology in post-mortem brain tissue. BRM has the capacity to provide rapid myelin imaging, which will provide information complementary to other myelin imaging techniques.

Approach

BRM enables label-free structural imaging of myelin with high spatial

SUBMITTER: Gray AJ 

PROVIDER: S-EPMC12576696 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature