Programmable Artificial-Cellular Membrane Dynamics via Ring-Closing Metathesis.

ABSTRACT: Lateral phase-separated structures in cellular membranes serve as functional domains in various membrane-mediated biological processes. Controlling phase-separation dynamics through catalysis could enable the assembly of smart vesicles that exhibit life-like phenomena. Herein, we present the first example of dynamic control of lateral phase separation via a catalytic reaction: ring-closing olefin metathesis (RCM). This catalytic reaction occurs near the membrane by engineering the surface of lipid membranes with a biotin-streptavidin-based artificial metalloenzyme (ArM). We designed a substrate that, following RCM, releases decanoic acid, which could be spontaneously incorporated into the lipid bilayer, resulting in the disappearance of lipid domains within the membrane. Genetic optimizati

SUBMITTER: Hamaguchi R 

PROVIDER: S-EPMC12576770 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature