Multivalency Enables Signal Processing at Single Protein Level.

ABSTRACT: Synthetic signal processing in cells can be achieved through complex networks of molecules or simpler, single-molecule-based mechanisms. The latter approach stands out for its reduced genetic load and ease of delivery. Multivalent protein binders are such single-molecule signal processors that exhibit distinct behaviors when interacting with cells presenting various antigen profiles. However, a quantitative and precise method to design multivalent interactions in the context of cell surface binding is still lacking. Here, we developed Multivalent Antigen Sensing Simulator (MASS) to guide the design of such multivalent proteins. We verified the model through in vitro and cell binding experiments, showing the predictive accuracy of our model in designing multivalent binders to sense c

SUBMITTER: Wu X 

PROVIDER: S-EPMC12576817 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature