Response to Nutrient Stress in the Industrial Model Bacterium Cupriavidus necator: A Thermal Proteome Profiling (TPP) Investigation.

ABSTRACT: The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources, or of autotrophic growth using CO₂ fixation with H₂ as an energy source. Under stress conditions, it produces biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total biomass. This metabolic versatility means that C. necator is under intense study for sustainable biotechnology processes; however, a relative lack of understanding of the overall regulatory architecture has limited its application. The major mechanisms by which proteins can respond to shifting cellular demands are protein expression change and/or allosteric regulation. Here, we use two powerful proteomics methods to investigate

SUBMITTER: McKeever K 

PROVIDER: S-EPMC12576926 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature