The stringent response does not influence ribosome pausing in Bacillus subtilis.

ABSTRACT: The stringent response represses translation and is activated when cells enter the stationary phase and intracellular amino acid levels drop. Bacillus subtilis, a well-known industrial enzyme production organism, secretes most enzymes during the stationary phase. However, translation pausing can affect protein folding and production. Here, we examined whether the stringent response affects ribosome pausing in B. subtilis. To investigate this, genome-wide ribosome profiling was performed using a stringent response mutant that overexpressed the α-amylase AmyM. Blocking the stringent response did increase overall protein synthesis, however AmyM production was reduced. Ribosome profiling revealed that this was not caused by a reduction in translation. In fact, absence of the stringent response

SUBMITTER: Han Y 

PROVIDER: S-EPMC12576955 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature