Cell deformability enhances adhesion of ATRA-differentiated HL-60 cells to P-selectin.

ABSTRACT: Acute promyelocytic leukemia (APL) is characterized by immature myeloid cells with unlimited proliferative capacity. All-trans retinoic acid (ATRA) can induce these cells to differentiate, but patients may develop differentiation syndrome (DS), a life-threatening complication linked to abnormal leukocyte recruitment. The mechanisms of DS remain poorly understood, despite insights gained from studies on APL cell lines. In this study, ATRA-differentiated HL-60 cells were perfused into a flow chamber simulating the physiological environment and functionalized with P-selectin to mimic the initial step of leukocyte recruitment. We quantified the adhesive behavior of these cells by measuring the PSGL-1-P-selectin bond lifetime and adhesion rate with and without ATRA treatment. Both parameters in

SUBMITTER: Luo X 

PROVIDER: S-EPMC12577115 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature