Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters.

ABSTRACT: Active transport of chemical species across the cell membrane represents a critical biological and biotechnological function, allowing the cell to selectively import compounds of nutritional value whilst exporting potentially toxic compounds. Major facilitator superfamily (MFS) transporters represent a ubiquitous class able to uptake and export an array of different chemical species. When designing biosynthetic pathways within microbial hosts, for production or remediation, transport is often critical to the efficiency of the resulting engineered strain. However, transport is a commonly neglected node for characterisation and engineering given difficulties in producing, purifying and assaying membrane transport proteins outside of their native environment. Here, using syntenic analysis and

SUBMITTER: Roy PL 

PROVIDER: S-EPMC12577307 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature