Prevalence of occult hepatitis B infection and hepatitis B genotype characterization among blood donors in Jember Regency, East Java, Indonesia.

ABSTRACT:

Background

Despite extensive HBV vaccination efforts in Indonesia, the prevalence of occult hepatitis B virus infection (OBI) remains a concern, particularly among blood donors. This study investigated the prevalence of OBI, the distribution of circulating HBV genotypes, seropositivity for HBV-specific antibodies, and the potential influence of mutational characteristics on HBsAg secretion among blood donors in Jember, Indonesia.

Methods

A total of 330 HBsAg-negative blood donor samples from the Indonesian Red Cross in Jember were analyzed for HBV serological markers. Qualitative nested PCR and quantitative real-time PCR were used to detect HBV viral DNA and to measure viral load, respectively. HBV genotyping in positive samples was performed using Sanger sequencing of the S

SUBMITTER: Sugiharto VD 

PROVIDER: S-EPMC12577327 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature