Lineage tracing and characterization of insulin-secreting cells generated from adult pancreatic acinar cells.

ABSTRACT: Although several studies have suggested that insulin-secreting cells can be generated in vitro from cells residing in adult exocrine pancreas, neither the origin of these cells nor their precise insulin secretory properties was obtained. We show here that insulin-secreting cells can be derived from adult mouse pancreatic exocrine cells by suspension culture in the presence of EGF and nicotinamide. The frequency of insulin-positive cells was only 0.01% in the initial preparation and increased to approximately 5% in the culture conditions. Analysis by the Cre/loxP-based direct cell lineage tracing system indicates that these newly made cells originate from amylase/elastase-expressing pancreatic acinar cells. Insulin secretion is stimulated by glucose, sulfonylurea, and carbachol, and potenti

SUBMITTER: Minami K 

PROVIDER: S-EPMC1257737 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature