The CBS/H2S axis regulates intestinal stem cell homeostasis and radiation-induced intestinal damage.

ABSTRACT:

Background

The cycling intestinal stem cells (ISCs) exhibit radiosensitivity, and their death or impaired regenerative capacity following irradiation may result in intestinal barrier dysfunction. The cystathionine-β-synthase (CBS)/H2S axis plays a critical role in regulating cell proliferation, reactive oxygen species scavenging, and the DNA damage response. However, it remains unclear whether the CBS/H2S axis modulates ISC homeostasis and tissue radiosensitivity.

Methods

Intestinal epithelium specific conditional CBS knockout mice were generated by crossing CBS^fl/+ mice with Villin-CreERT2 mice. CAGGCre-ER™ mice were crossed with CBS^fl/fl mice to achieve CBS knockout in multiple tissues and cell types. The Lgr5-Tdtaomato-Flag mice were generated by CRI

SUBMITTER: Wu T 

PROVIDER: S-EPMC12577406 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature