Assessing the Independent and Combined Effects of Hyperosmolarity and Inflammatory Stress on Human Corneal Epithelial Cells.

ABSTRACT:

Purpose

Inflammation and hyperosmolarity drive dry eye disease (DED) by inducing oxidative stress, apoptosis, and immune activation, disrupting tear film homeostasis and epithelial integrity. This study aimed to evaluate the independent and combined effects of hyperosmolarity and inflammatory stress on human corneal epithelial (HCE) cells.

Methods

HCE cells were exposed to hyperosmolar conditions using sodium chloride, lipopolysaccharide, or a combination of both for 12, 24, and 48 hours. The effects on cell adhesion, cell proliferation, cytokine secretion, and gene expression were analyzed. Expression of genes and inflammatory cytokines was evaluated for stress response pathways and antioxidant marker regulation.

Results

LPS exposure alone significantly increased inf

SUBMITTER: Prasad D 

PROVIDER: S-EPMC12577738 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature