Multiplex PCR assay for the rapid detection of <i>Klebsiella pneumoniae</i> pathotypes.

ABSTRACT: Introduction. Klebsiella pneumoniae (Kp) is a major cause of nosocomial infections, with its evolving pathotypes including multidrug-resistant, hypervirulent (hvKp) and convergent strains posing significant diagnostic and treatment challenges due to combined antimicrobial resistance and virulence.Gap Statement. While there is a pressing requirement for thorough detection of Kp pathotypes, current assays in resource-limited environments are unable to effectively focus on essential carbapenemase and hypervirulence genes with the necessary reliability and precision.Aim. To develop and validate a multiplex PCR (m-PCR) assay capable of simultaneously detecting Kp isolates including those carrying partial or full virulence markers, alongside antimicrobial resistance.<

SUBMITTER: Kulkarni SM 

PROVIDER: S-EPMC12578128 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature