Micro-Computed Tomography as a complementary tool for histopathological diagnosis of oral soft tissue lesions - Proof of concept.

ABSTRACT:

Background

Accurate diagnosis of oral soft tissue lesions is critical for effective treatment, yet conventional histopathological examination, the gold standard, faces limitations. These include two-dimensional (2D) visualization and malorientation, which can obscure critical diagnostic features, like epithelial-connective tissue interfaces. Micro-computed tomography (µCT) offers a non-destructive, high-resolution three-dimensional (3D) imaging alternative to address these challenges. Still, its use for soft tissue visualization is limited. We tested a method with specific radio-opaque staining and µCT scanning settings to visualize oral soft tissue biopsies as a proof of concept.

Methods

Biopsies from 12 patients with different oral mucosa lesions were stained with Lugol's

SUBMITTER: Kats L 

PROVIDER: S-EPMC12578140 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature