Optimizing blood alcohol concentration measurement with breath alcohol meter and its preliminary application in evaluating CYP2E1 activity.

ABSTRACT:

Background

Ethanol metabolism in vivo is primarily mediated by CYP2E1 and alcohol dehydrogenase, with CYP2E1 playing the predominant role at high ethanol concentrations. However, it is a difficult problem to evaluate CYP2E1 activity with ethanol as a probe, especially in rats. Currently, high-performance liquid chromatography (HPLC)-based detection of chlorzoxazone is the standard method for evaluating CYP2E1 activity. Nevertheless, this method is labor-intensive, time-consuming, costly, and unsuitable for real-time on-site or multipoint noninvasive monitoring.

Objectives

This study aimed to develop and optimize a noninvasive and rapid method for blood alcohol concentration BAC detection in rats. The optimized approach was subsequently applied to preliminarily assess hepatic

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12578163 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature