METTL3 facilitates the translation of CircSIK2 during chicken myogenesis in an m6A dependent manner.

ABSTRACT: Circular RNAs (circRNAs) are generally considered a new class of non-coding RNA (ncRNA) that frequently appears in the eukaryotic transcriptome. In principle, circRNAs may encode proteins, as some of them are generated from exons and possess elements for internal ribosome entry. Circular RNAs have the potential to serve as an unexplored reservoir for the generation of novel proteins, yet the identification of coding-circRNAs is a daunting task. In this study, we developed a specialized strategy for the discovery of coding-circRNA by combining RNA sequencing, ribosome profiling, and mass spectrometry to find a multitude of circRNAs translated in vivo. A total of 40,084 circRNAs were found in chicken myoblasts and myotubes, and 15,332 circRNAs had a predicted open reading frame (ORF). Via ri

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12578262 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature