Investigating the Role of Zebrafish Retinoschisin Homologs Rs1a and Rs1b During Retinal Development.

ABSTRACT: Deficiency in the retinoschisin protein (RS1) causes X-linked juvenile retinoschisis (XLRS), a retinal degenerative disease that disrupts retinal layers and forms cystic cavities. In addition to its structural function, RS1 is believed to play a role in retinal development. A zebrafish model may provide insights into the role of Rs1 in the earliest stages of retinal development. To explore this, we created a zebrafish model with RS1 deficiency by knocking down the two homologs, Rs1a and Rs1b. Gene expression and protein presence were assessed in Wildtype Tüpfel Longfin zebrafish at 1, 24, 48, 72, 96, and 120 h post-fertilization (hpf). We then performed morpholino (MO)-mediated knockdown targeting rs1a and rs1b mRNA, using scrambled oligos (SC) as controls. MOs or SCs were injected at the

SUBMITTER: van der Veen I 

PROVIDER: S-EPMC12578269 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature