Luminal LPS is transported across murine small intestine by a caveolin-1 dependent endocytic mechanism induced by chronic feeding of a Western-style diet.

ABSTRACT: Metabolic endotoxemia is an important risk factor for the metabolic syndrome (MetS). We report that lipopolysaccharides (LPS) are transcellularly transported from the jejunal lumen during long-chain fatty acids (LCFA) absorption via caveolin (Cav)-1-mediated endocytosis. Here, we examined the effects of diets of varying fatty acid (FA) composition on the induction of MetS and LPS transport. We fed wild-type (WT) and Cav-1 knockout (KO) mice with a standard diet (SD), or one of three high-fat/high-sucrose diets enriched with saturated LCFA, medium-chain FA, or polyunsaturated FA, measuring FITC-LPS or FITC-dextran (FD4) mucosa-to-serosa transport in the jejunum and colon. MetS rapidly developed in the male WT LCFA diet group with increased portal endotoxemia, less so in WT other dietary gro

SUBMITTER: Akiba Y 

PROVIDER: S-EPMC12578307 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature