The TRAPPIII complex regulates development and virulence of Fusarium graminearum by coordinating autophagy and intracellular transport.

ABSTRACT: TRAnsport Protein Particle (TRAPP) is a conserved multi-subunit tethering complex known to be involved in intracellular movement of proteins. However, its components and molecular functions in filamentous fungi remain poorly characterized. Here, we identify four TRAPPIII-specific subunits (FgTrs85, TRAPPC11, TRAPPC12, and TRAPPC13) in the phytopathogenic fungus Fusarium graminearum. Genetic and functional analyses reveal that FgTrs85 serves as the core subunit, collaborating with auxiliary subunits TRAPPC11, TRAPPC12, and TRAPPC13 to orchestrate fungal perithecium formation, growth, and virulence. TRAPPIII localizes to the phagophore assembly site and promotes autophagosome biogenesis by recruiting FgAtg9 through interactions involving FgTrs85 and TRAPPC13. Notably, TRAPPIII mutants exhibi

SUBMITTER: Chen L 

PROVIDER: S-EPMC12578332 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature