Identification of C1QA as a prognostic marker and regulator of immunosuppressive neutrophils in early-stage lung adenocarcinoma through integrated bioinformatics analyses.

ABSTRACT: Neutrophils infiltrate lung tumours and can exhibit an immunosuppressive phenotype that promotes tumour growth, yet their regulation in early-stage lung cancer remains unclear. This study investigates molecular regulators of neutrophils in early-stage lung adenocarcinoma (LUAD) using publicly available gene expression datasets. An early-stage LUAD dataset (GSE31210) was analysed using CIBERSORTx to estimate neutrophil abundance. Weighted gene co-expression network analysis (WGCNA) identified the hub gene most correlated with neutrophil scores. Single-cell RNA sequencing (scRNA-seq) was used to determine the cellular source and regulatory mechanisms of this gene. A high neutrophil score was a negative prognostic factor, validated in independent datasets. WGCNA identified C1QA as the key gen

SUBMITTER: Nguyen QA 

PROVIDER: S-EPMC12578707 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature