A multiplex real-time PCR assay for detection of equid herpesvirus 1 and 4.

ABSTRACT: Equid herpesvirus (EHV) 1 and -4 are common viral pathogens of horses that can cause upper respiratory disease, neurological disease, abortion, and death. As characteristic alphaherpesviruses, both EHV-1 and EHV-4 can establish latency, resulting in a lifelong carrier state in infected animals. Here we describe the development and validation of a rapid and sensitive multiplex real-time PCR assay (EHV1-4MP) that simultaneously detects EHV-1 and EHV-4 and includes an endogenous internal control - melanocortin 1 receptor (MC1R) - targeting the equid genome. The EHV1-4MP assay analytical sensitivity was determined to be approximately two copies for EHV-1, four copies for EHV-4, and 10 copies for the equid MC1R gene per reaction. Analytical specificity was determined using a panel of 28 equine

SUBMITTER: Tallmadge RL 

PROVIDER: S-EPMC12578806 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature