Long-read methylome analysis of Oleidesulfovibrio alaskensis G20 biofilm under copper stress.

ABSTRACT: This study represents the first investigation of 5-methyl cytosine (5mC) DNA methylation patterns in sulfate-reducing bacterial (SRB) biofilms under copper (Cu) stress, utilizing Oxford Nanopore Technologies (ONT) sequencing. DNA methylation is a crucial epigenetic modification that is dynamic and regulates the signals to modulate molecular mechanisms across biological systems. The regulatory roles of DNA methylation in prokaryotic systems remain comparatively understudied than in eukaryotes. Bacteria are highly sensitive to environmental changes and therefore may utilize additional mechanisms like DNA methylation to combat the stresses. Our previous studies, utilizing microscopy and growth analyses, revealed that Oleidesulfovibrio alaskensis G20 (OA G20) biofilms responded to Cu stress. H

SUBMITTER: Thakur P 

PROVIDER: S-EPMC12579266 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature