Mechanical stretching enhances the cellular and paracrine effects of bone marrow mesenchymal stem cells on diabetic wound healing.

ABSTRACT:

Background

Diabetic wounds present persistent clinical challenges characterized by disrupted extracellular matrix (ECM) homeostasis, which critically impedes tissue regeneration. While bone marrow-derived mesenchymal stem cells (BMSCs) exhibit therapeutic potential through ECM remodeling, conventional transplantation strategies are limited by suboptimal cell retention and transient therapeutic effects.

Methods

BMSCs cultured on Flexcell plates were subjected to programmable mechanical stretching using a custom-built spherical cell-stretching system. Strain rate- and duration-dependent effects on paracrine signaling and ECM secretion were longitudinally assessed through western blot ting and ELISA. The optimized mechanical parameters (15% deformation, 1440 cycles, 5-s vertex

SUBMITTER: Dai W 

PROVIDER: S-EPMC12579390 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature