Spatially Mapping Neuropeptide Isomers via MALDI Trapped Ion Mobility MS Imaging.

ABSTRACT: Imaging endogenous peptides with the chiral selectivity of their component amino acids remains an unmet analytical challenge. d-amino acid-containing peptides (DAACPs), formed by post-translational isomerization of l- to d-amino acids, make up a functionally important class of neuropeptides whose spatial distribution remains poorly understood. Here, we introduce untargeted imaging of neuropeptide stereoisomers using matrix-assisted laser desorption/ionization-trapped ion mobility mass spectrometry imaging (MALDI-TIMS-MSI). We mapped neuropeptides and their DAACP forms in the central nervous system (CNS) of Aplysia californica at single-cell resolution. We found that while both stereoisomeric forms of known neuropeptides were colocalized in nerves and neuropil, DAACPs were not detect

SUBMITTER: Okyem S 

PROVIDER: S-EPMC12579433 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature