Cytokine supplementation influences bovine embryo transcriptome during the preimplantation period.

ABSTRACT:

Abstract

The central goal of the following studies was to understand how FGF2, LIF, and IGF1, a cocktail called 'FLI', influence bovine embryo development by the degree of transcriptomic variation throughout preimplantation development. All embryos were produced in vitro with or without FLI supplementation at the beginning of culture. For each treatment, embryos were collected at the 4-6 cell, 9-16 cell, morula, or blastocyst stages, and RNA was isolated and sequenced at a depth of 50 million reads per sample. In the FLI group, at the 9-16 cell stage, there were seven upregulated and six downregulated differentially expressed genes (DEGs). At the morula stage, of the 1,856 DEGs, 580 were upregulated in FLI. Gene ontology analysis showed increased MAPK signaling, TGF-beta signaling,

SUBMITTER: McDonald KS 

PROVIDER: S-EPMC12579512 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature