Spatial protein profiling reveals active roles for astrocytes in the chronic active lesion core during multiple sclerosis.

ABSTRACT: Chronic active lesions are a manifestation of multiple sclerosis (MS) and have been associated with disease progression. While astrocytes are heavily implicated in MS, little is known about their role in lesions, particularly in the lesion core. Here, we sought to gain insight into the spatial relationship between astrocytes and defined regions of chronic active lesions, and to better understand the environment within the relatively understudied lesion core, an area primarily composed of astrocytes. We analyzed four defined protein panels, focusing on astrocytes, in postmortem fresh-frozen cortical white matter tissue using NanoString GeoMx spatial protein profiling to compare normal appearing white matter (NAWM), the chronic active perilesion, rim, and core. We then performed immunofluore

SUBMITTER: Smith BC 

PROVIDER: S-EPMC12579715 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature