Single-Cell Transcriptomic Analysis Identifies a Novel OLR1⁺ SLC7A7⁺ Liver-Enriched Metastatic Subset With Immunometabolic Rewiring in Pancreatic Cancer.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) is an exceptionally lethal malignancy, with high percents of patients presenting with liver metastases (LM). However, the mechanisms driving liver metastases remain critical bottlenecks requiring urgent exploration.

Objective

To identify the key cellular subsets driving PDAC liver metastases, elucidate their interactions with the metastatic microenvironment, and define the underlying mechanisms of liver colonization.

Materials and methods

Integrated single-cell transcriptomic analysis was performed using scRNA-seq data of PT and LM. The expression of signature genes within the identified cell subset was validated using clinical samples from PDAC PT and LM patients. Furthermore, ligand-receptor network analysis was con

SUBMITTER: Yang X 

PROVIDER: S-EPMC12579899 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature