RhoGEF2 overexpression induces cell competition dependent on Ptp10D, Crumbs and the Hippo signaling pathway.

ABSTRACT: In Drosophila melanogaster larval epithelial tissues, cells containing mutations in the apico-basal polarity protein, Scrib, are eliminated by cell competition when surrounded by wild-type cells. In scrib mutant cells, signaling mediated by the receptor-type tyrosine phosphatase Ptp10D upon engagement with its ligand Sas in the surrounding wild-type cells triggers cell competition via EGFR-Ras pathway inhibition and JNK pathway activation, which induces apoptosis of the mutant cells. Here, we investigate whether overexpression of RhoGEF2 (RhoGEF2OE), which induces Rho signaling and affects actin cytoskeleton regulators, acts similarly to Scrib depletion in cell competition. We show that RhoGEF2OE cells are eliminated when surrounded by wild-type cells and that Ptp10D knockdown increases Rh

SUBMITTER: Fahey-Lozano N 

PROVIDER: S-EPMC12579956 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature