Nanoscale architecture and dynamics of Ca_V1.3 channel clusters in cardiac myocytes revealed by single channel nanoscopy.

ABSTRACT: The clustering of L-type calcium channels (LTCC) for functional regulation of intracellular calcium signaling remains poorly understood. Here we applied super-resolution imaging to study Ca_V1.3 channel clusters in human iPSC-derived atrial cardiomyocytes (hiPSC-aCM) to analyze subcellular localization, dimensions, architecture, and dynamics, which were largely unexplored previously. STimulated Emission Depletion (STED) imaging characterized the localization and structure of Ca_V1.3 channel clusters in living cardiomyocytes. DNA Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) achieved true molecular resolution, revealing an irregular channel distribution with substantial spacing. Single Particle Tracking (SPT) showed that channels co-diffuse in confi

SUBMITTER: Schwenzer N 

PROVIDER: S-EPMC12580110 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature