Identifying overtraining biomarkers through proteomic analysis of extracellular vesicles derived from the central nervous system of male mice.

ABSTRACT: Overtraining, which results in central nervous system (CNS)-fatigue-related symptoms, leads to a long-term decline in performance. This study investigated CNS-derived EV protein content under overtraining conditions to identify potential biomarkers. Eight-week-old C57BL/6J mice were randomly divided into three groups: sedentary (SED, n = 7), exercise control (EX, n = 8), and overtraining (OT, n = 9) groups. The OT group underwent an 8-week downhill treadmill-based overtraining induction protocol. Exercise capacity was assessed using the incremental load, exhaustion, grip strength, and rotarod tests, while motor deficits, depression and anxiety were assessed using the nest building test. Proinflammatory cytokines were measured in blood plasma, skeletal muscle, and brain tissue. CNS-derived

SUBMITTER: Yi DH 

PROVIDER: S-EPMC12580405 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature