Discovering Heterogeneous Leukocytes Subsets Associated With Alcoholic Steatohepatitis by scRNAseq Analysis.

ABSTRACT: The precise identification of immune cell type responses to alcoholic steatohepatitis (ASH) at the single-cell level remains unresolved. Therefore, in this study, we analyzed heterogeneous immune leukocytes associated with ASH at the single-cell level using high-dimensional single-cell RNA sequencing in alcoholic liver disease (ALD)-induced and healthy control mice. A t-distributed stochastic neighbor embedding plot for dimensionality reduction and 2D visualization was used to visualize heterogeneous immune cell types. Moreover, singleR was used for automated cell annotation to identify the cell types and differentially expressed genes from each cell type and their subsets. We observed a decline in the population of B cells and their subsets, with up and downregulated genes signifying an i

SUBMITTER: Perumalsamy H 

PROVIDER: S-EPMC12580407 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature