Retrospective transcriptomic analysis indicates temporal dysregulation of mitochondrial genes and metabolic pathways after volumetric muscle loss injury.

ABSTRACT: Volumetric muscle loss (VML) injury results in the irrecoverable loss of muscle mass and strength and alters the metabolic capacity of the remaining muscle tissue. The primary objective of this retrospective study was to leverage existing RNA-seq datasets to investigate mitochondria and metabolic transcriptome changes after VML injury. The datasets were extracted from publicly available sources and included a bulk RNA-seq dataset (Rattus norvegicus) and a single-cell RNA-seq dataset (Mus musculus) that combined provided a transcriptional landscape out to 42 days post-injury (dpi). The Broad Institute's MitoCarta3.0 database was used to identify mitochondrial-associated genes and pathways for the analysis. There was a robust downregulation of genes in the bulk RNA-seq dataset out to 28 dpi.

SUBMITTER: Miller DL 

PROVIDER: S-EPMC12580409 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature