Quantitative single-molecule FLIM and PIE-FRET imaging of biomolecular systems.

ABSTRACT:

Background

The structural dynamics of proteins and nucleic acids are critical for their function in many biological processes but investigating these dynamics is often challenging with traditional techniques. Time-correlated single photon counting (TCSPC) coupled with confocal microscopy is a versatile biophysical tool that enables real-time monitoring of biomolecular dynamics in a variety of systems, across many timescales. Quantitative single-molecule time-resolved fluorescence methods are uniquely positioned to investigate transient interactions and structural changes, yet application in complex biological systems remains limited by technical and analytical challenges. Combining fluorescence lifetime imaging microscopy (FLIM) with pulsed interleaved excitation Förster resonance

SUBMITTER: Silvernail I 

PROVIDER: S-EPMC12580441 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature